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Image Search Results
Journal: Oncology Reports
Article Title: miR-199a-3p enhances cisplatin sensitivity of ovarian cancer cells by targeting ITGB8
doi: 10.3892/or.2018.6259
Figure Lengend Snippet: ITGB8 is a direct target of miR-199a-3p. (A) Sequence alignment of two putative miR-199a-3p binding sites in the 3′-UTR of ITGB8. (B) The protein levels of ITGB8 in SKOV3/CDDP and SKOV3 cells examined by western blotting. (C) Relative luciferase activities of plasmids carrying ITGB8 3′-UTR-1 or ITGB8 3′-UTR-2, or both in SKOV3/CDDP cells co-transfected with miR-199a-3p mimics. Data shown are mean ± SD, ***P<0.001, compared with control mimic (one-way ANOVA with Dunett's test). (D) Relative luciferase activities of plasmids carrying WT or mutant of ITGB8 3′-UTR-2 in SKOV3/CDDP cells co-transfected with miR-199a-3p mimics or miR-199a-3p inhibitor. Data shown are mean ± SD, ***P<0.01, compared with inhibitor (one-way ANOVA with Dunett's test). (E) The expression levels of ITGB8 in SKOV3/CDDP cells were examined by real-time PCR and western blotting. Data shown are mean ± SD, P<0.01, compared with miR-NC (Student's t-test).
Article Snippet: The membrane was incubated with primary
Techniques: Sequencing, Binding Assay, Western Blot, Luciferase, Transfection, Control, Mutagenesis, Expressing, Real-time Polymerase Chain Reaction
Journal: Oncology Reports
Article Title: miR-199a-3p enhances cisplatin sensitivity of ovarian cancer cells by targeting ITGB8
doi: 10.3892/or.2018.6259
Figure Lengend Snippet: Overexpression of ITGB8 enhances CDDP resistance in miR-199a-3p overexpressed ovarian cancer cells. SKOV3/CDDP/miR-199a-3p cells were transfected with pcDNA3.1-ITGB8 plasmid. (A) The transfection efficiency was validated by western blotting. (B) Cell proliferation was detected by CCK-8 assay. (C) Cell apoptosis was detected by Annexin V-FITC/PI detection kit. (D) Cell cycle distribution was detected by PI staining and flow cytometry. (E) Cell invasion was measured using the Transwell assay. Data shown are the representative images and quantitative data. Data shown are mean ± SD, *P<0.05, **P<0.01, compared with vector (Student's t-test).
Article Snippet: The membrane was incubated with primary
Techniques: Over Expression, Transfection, Plasmid Preparation, Western Blot, CCK-8 Assay, Staining, Flow Cytometry, Transwell Assay